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af2000 asymmetric flow field flow fractionation af4 module  (Postnova Analytics)

 
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    Postnova Analytics af2000 asymmetric flow field flow fractionation af4 module
    <t>FI-AF4-MD</t> of Poly­(A) LNP formulations. MALS-90° elution profiles with FWHM-integrated radius of gyration and hydrodynamic radii for (A) A-LNPs, (B) B-LNPs, and (C) C-LNPs ( n = 3 ± SD). Cumulative distributions for (D) radius of gyration, (E) hydrodynamic radius, and (F) shape factor ( n = 3), with respective 10, 50, and 90 distributional values for (G) radius of gyration, (H) hydrodynamic radius, and (I) shape factor for A-LNPs, B-LNPs and C-LNPs ( n = 3 ± SD).
    Af2000 Asymmetric Flow Field Flow Fractionation Af4 Module, supplied by Postnova Analytics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/af2000 asymmetric flow field flow fractionation af4 module/product/Postnova Analytics
    Average 86 stars, based on 1 article reviews
    af2000 asymmetric flow field flow fractionation af4 module - by Bioz Stars, 2026-05
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    1) Product Images from "The Use of Enhanced Analytical Pipelines for the Characterization of Poly(A) and Poly(A)-LNP Formulation Critical Quality Attributes"

    Article Title: The Use of Enhanced Analytical Pipelines for the Characterization of Poly(A) and Poly(A)-LNP Formulation Critical Quality Attributes

    Journal: Molecular Pharmaceutics

    doi: 10.1021/acs.molpharmaceut.5c00614

    FI-AF4-MD of Poly­(A) LNP formulations. MALS-90° elution profiles with FWHM-integrated radius of gyration and hydrodynamic radii for (A) A-LNPs, (B) B-LNPs, and (C) C-LNPs ( n = 3 ± SD). Cumulative distributions for (D) radius of gyration, (E) hydrodynamic radius, and (F) shape factor ( n = 3), with respective 10, 50, and 90 distributional values for (G) radius of gyration, (H) hydrodynamic radius, and (I) shape factor for A-LNPs, B-LNPs and C-LNPs ( n = 3 ± SD).
    Figure Legend Snippet: FI-AF4-MD of Poly­(A) LNP formulations. MALS-90° elution profiles with FWHM-integrated radius of gyration and hydrodynamic radii for (A) A-LNPs, (B) B-LNPs, and (C) C-LNPs ( n = 3 ± SD). Cumulative distributions for (D) radius of gyration, (E) hydrodynamic radius, and (F) shape factor ( n = 3), with respective 10, 50, and 90 distributional values for (G) radius of gyration, (H) hydrodynamic radius, and (I) shape factor for A-LNPs, B-LNPs and C-LNPs ( n = 3 ± SD).

    Techniques Used:



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    <t>FI-AF4-MD</t> of Poly­(A) LNP formulations. MALS-90° elution profiles with FWHM-integrated radius of gyration and hydrodynamic radii for (A) A-LNPs, (B) B-LNPs, and (C) C-LNPs ( n = 3 ± SD). Cumulative distributions for (D) radius of gyration, (E) hydrodynamic radius, and (F) shape factor ( n = 3), with respective 10, 50, and 90 distributional values for (G) radius of gyration, (H) hydrodynamic radius, and (I) shape factor for A-LNPs, B-LNPs and C-LNPs ( n = 3 ± SD).
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    Fig. 1. Schematic showing formation (A) Lipid nanoparticles (LNP) components with difference in ionisable lipids (D-Lin-MC3-DMA referred as MC3 and SM-102), (B) Side-view of frit-inlet <t>AF4</t> channel with protein, LNP and LNP-protein corona in the channel and (C) a representative simulated AF4 profile of Bovine Serum Albumin (BSA) and LNP with the applied cross-flow (XFlow) and detector signal (A) and (B) created with BioRender.com and (C) NovaAnalysis software.
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    Fig. 1. Schematic showing formation (A) Lipid nanoparticles (LNP) components with difference in ionisable lipids (D-Lin-MC3-DMA referred as MC3 and SM-102), (B) Side-view of frit-inlet <t>AF4</t> channel with protein, LNP and LNP-protein corona in the channel and (C) a representative simulated AF4 profile of Bovine Serum Albumin (BSA) and LNP with the applied cross-flow (XFlow) and detector signal (A) and (B) created with BioRender.com and (C) NovaAnalysis software.
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    Fig. 1. Schematic showing formation (A) Lipid nanoparticles (LNP) components with difference in ionisable lipids (D-Lin-MC3-DMA referred as MC3 and SM-102), (B) Side-view of frit-inlet <t>AF4</t> channel with protein, LNP and LNP-protein corona in the channel and (C) a representative simulated AF4 profile of Bovine Serum Albumin (BSA) and LNP with the applied cross-flow (XFlow) and detector signal (A) and (B) created with BioRender.com and (C) NovaAnalysis software.
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    Fig. 1. Schematic showing formation (A) Lipid nanoparticles (LNP) components with difference in ionisable lipids (D-Lin-MC3-DMA referred as MC3 and SM-102), (B) Side-view of frit-inlet <t>AF4</t> channel with protein, LNP and LNP-protein corona in the channel and (C) a representative simulated AF4 profile of Bovine Serum Albumin (BSA) and LNP with the applied cross-flow (XFlow) and detector signal (A) and (B) created with BioRender.com and (C) NovaAnalysis software.
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    Image Search Results


    FI-AF4-MD of Poly­(A) LNP formulations. MALS-90° elution profiles with FWHM-integrated radius of gyration and hydrodynamic radii for (A) A-LNPs, (B) B-LNPs, and (C) C-LNPs ( n = 3 ± SD). Cumulative distributions for (D) radius of gyration, (E) hydrodynamic radius, and (F) shape factor ( n = 3), with respective 10, 50, and 90 distributional values for (G) radius of gyration, (H) hydrodynamic radius, and (I) shape factor for A-LNPs, B-LNPs and C-LNPs ( n = 3 ± SD).

    Journal: Molecular Pharmaceutics

    Article Title: The Use of Enhanced Analytical Pipelines for the Characterization of Poly(A) and Poly(A)-LNP Formulation Critical Quality Attributes

    doi: 10.1021/acs.molpharmaceut.5c00614

    Figure Lengend Snippet: FI-AF4-MD of Poly­(A) LNP formulations. MALS-90° elution profiles with FWHM-integrated radius of gyration and hydrodynamic radii for (A) A-LNPs, (B) B-LNPs, and (C) C-LNPs ( n = 3 ± SD). Cumulative distributions for (D) radius of gyration, (E) hydrodynamic radius, and (F) shape factor ( n = 3), with respective 10, 50, and 90 distributional values for (G) radius of gyration, (H) hydrodynamic radius, and (I) shape factor for A-LNPs, B-LNPs and C-LNPs ( n = 3 ± SD).

    Article Snippet: Analytical SEC (aSEC) was performed on the AF2000 Asymmetric-Flow Field-Flow Fractionation (AF4) module (Postnova Analytics, Germany), hyphenated with multiple inline detectors (as per ), using a TOSOH TSKgel UP-SW2000 column (300 × 4.6 mm, 2 μm) and 1× PBS pH 7.4 isocratic mobile phase with a corresponding flow rate of 0.2 mL/min and equivalent injection concentration to that in .

    Techniques:

    Fig. 1. Schematic showing formation (A) Lipid nanoparticles (LNP) components with difference in ionisable lipids (D-Lin-MC3-DMA referred as MC3 and SM-102), (B) Side-view of frit-inlet AF4 channel with protein, LNP and LNP-protein corona in the channel and (C) a representative simulated AF4 profile of Bovine Serum Albumin (BSA) and LNP with the applied cross-flow (XFlow) and detector signal (A) and (B) created with BioRender.com and (C) NovaAnalysis software.

    Journal: Journal of chromatography. A

    Article Title: Frit-inlet asymmetric flow field-flow fractionation for the analysis of lipid nanoparticle-protein interactions.

    doi: 10.1016/j.chroma.2025.465663

    Figure Lengend Snippet: Fig. 1. Schematic showing formation (A) Lipid nanoparticles (LNP) components with difference in ionisable lipids (D-Lin-MC3-DMA referred as MC3 and SM-102), (B) Side-view of frit-inlet AF4 channel with protein, LNP and LNP-protein corona in the channel and (C) a representative simulated AF4 profile of Bovine Serum Albumin (BSA) and LNP with the applied cross-flow (XFlow) and detector signal (A) and (B) created with BioRender.com and (C) NovaAnalysis software.

    Article Snippet: A Postnova Analytics AF2000 asymmetric Flow Field-Flow Fractionation (AF4) (Landsberg, Germany) was used in this study.

    Techniques: Software

    Fig. 2. FI-AF4-UV-MALS fractograms for (A) MC3-LNPs (control) and MC3-LNPs incubated in 35 mg/mL Bovine Serum Albumin (BSA), (B) SM-102 LNPs, (C) MALS fractograms (90◦) plotted for peak 1 and peak 2 for MC3-LNPs incubated in 35 mg/mL BSA, (D) SM-102 LNPs. Time-points indicate 0 hour (control) and 24 h incubation at 37 ◦C. Error bars represent ± S.D mean of triplicate injections. The peaks marked with a red asterisk (*) show enlarged peaks. Vertical dashed lines indicate peak region of interest.

    Journal: Journal of chromatography. A

    Article Title: Frit-inlet asymmetric flow field-flow fractionation for the analysis of lipid nanoparticle-protein interactions.

    doi: 10.1016/j.chroma.2025.465663

    Figure Lengend Snippet: Fig. 2. FI-AF4-UV-MALS fractograms for (A) MC3-LNPs (control) and MC3-LNPs incubated in 35 mg/mL Bovine Serum Albumin (BSA), (B) SM-102 LNPs, (C) MALS fractograms (90◦) plotted for peak 1 and peak 2 for MC3-LNPs incubated in 35 mg/mL BSA, (D) SM-102 LNPs. Time-points indicate 0 hour (control) and 24 h incubation at 37 ◦C. Error bars represent ± S.D mean of triplicate injections. The peaks marked with a red asterisk (*) show enlarged peaks. Vertical dashed lines indicate peak region of interest.

    Article Snippet: A Postnova Analytics AF2000 asymmetric Flow Field-Flow Fractionation (AF4) (Landsberg, Germany) was used in this study.

    Techniques: Control, Incubation